5-6mpg is far, far, far too much to be attributed to running the a/c, IMHO.
I've never been able to discern a difference of any sort in my car, which has lots of ways to view the MPG (as graphs, etc.), or any of our cars for that matter. Most of my best tanks in the Camry (39 or better mpg) have involved significant a/c use. I'd be extraordinarily surprised if the difference even approached 1mpg in your Vibe. I'd say you either have another mechanical issue (say, a dragging caliper), a measurement error/issue (e.g. you had the first fill-up be short a gallon or so, and maybe the second was a gallon or so more, throwing off your numbers), or your driving style changed in some way (more traffic, idling, long drive-throughs, etc.) Or you could've gotten a tank of higher-ethanol gas accidentally (perhaps E-85 vs E-10). I've tracked every tank of gas going into my car, and watch the MPG readouts quite a bit, so have a pretty good sense of what impacts fuel mileage in my driving. Any of the other options could cause such a MPG drop, but a/c shouldn't even make a noticeable impact on your fuel economy, much less 6mpg.